| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1251 | Clostridium butyricum | GCGGTTCAACAGCTGCTTT | GCCAAGCCATGTTTCGTCC | 59.64 | 59.79 | 171 | 50 |
100.00%
|
50.00%
|
| Primer 1252 | Clostridium butyricum | AAAGGAGTTCAGCAGCTCTTG | GCACAAGGGGAGACAGCAT | 58.50 | 60.00 | 246 | 49 |
100.00%
|
50.00%
|
| Primer 1253 | Clostridium butyricum | AGGGAAGCAGGGGTGTCTAT | AGAAACGTCTTCTGCTCCTCT | 59.96 | 58.76 | 231 | 49 |
100.00%
|
50.00%
|
| Primer 1254 | Clostridium butyricum | CGTATTGGATTGGAATGGCGA | TGCAAGTGTTCCAAGTGATGC | 58.78 | 59.66 | 254 | 49 |
100.00%
|
50.00%
|
| Primer 1255 | Clostridium butyricum | CGTATTGGATTGGAATGGCGA | GCAAGTGTTCCAAGTGATGCT | 58.78 | 59.39 | 253 | 49 |
100.00%
|
50.00%
|
| Primer 1256 | Clostridium butyricum | ATTCACTGGCTTGAATGCGA | ACTTCATCCACGCCTGCAA | 58.17 | 59.93 | 164 | 49 |
100.00%
|
50.00%
|
| Primer 1257 | Clostridium butyricum | ATTCACTGGCTTGAATGCGA | AACTTCATCCACGCCTGCA | 58.17 | 59.93 | 165 | 49 |
100.00%
|
50.00%
|
| Primer 1258 | Clostridium butyricum | AAATGCCAGATGGTGCCGA | CAGCGCTTGAATCAACAGCT | 60.00 | 59.48 | 156 | 49 |
100.00%
|
50.00%
|
| Primer 1259 | Clostridium butyricum | AGTCCGGAGCAGCCCATAT | TCCTGGCCTGTACACATGC | 60.46 | 59.70 | 194 | 49 |
100.00%
|
50.00%
|
| Primer 1260 | Clostridium butyricum | TCGCCTCAATCAGCTGCA | TGGGCAACTCCTAAGGCGA | 59.34 | 60.92 | 188 | 49 |
100.00%
|
50.00%
|